In silico chromosome staining: Reconstruction of Giemsa bands from the whole human genome sequence

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In silico chromosome staining: reconstruction of Giemsa bands from the whole human genome sequence.

Giemsa staining has been used for identifying individual human chromosomes. Giemsa-dark and -light bands generally are thought to correspond to GC-poor and GC-rich regions; however, several experiments showed that the correspondence is quite poor. To elucidate the precise relationship between GC content and Giemsa banding patterns, we developed an "in silico chromosome staining" method for reco...

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The Giemsa-staining centromeres of Nigella damascena.

The centromere regions of each chromosome in the complement of Nigella damascena (2n equals 2x equals 12) stain differentially with Giemsa at interphase and throughout all the principal stages of mitosis and meiosis. Each centromere is seen to consist of a pair of sister half-centromeres which appear as 2 differentially stained dots. The appearance and behaviour of these dots indicates that the...

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Whole Genome Annotation: In Silico Analysis

After a genome is assembled, the next step is genomic annotation, which can generate data that will allow various types of research of the model organism. Complete DNA sequences of the organism are then mapped in areas pertinent to the research objectives. In this chapter, we explore relevant ongoing research on genes and consider the gene as a basic mapping unit. Gene prediction is the first h...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2002

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.022437999